Laura Necula

Latest posts by Laura Necula (see all)

EVALUAREA A PATRU TESTE FENOTIPICE PENTRU DETECTAREA CARBAPENEMAZELOR DE TIP OXA-48 LA ENTEROBACTERIACEAE - 14/02/2017
EVALUATION OF FOUR PHENOTYPIC TESTS FOR THE DETECTION OF OXA-48-TYPE CARBAPENEMASE IN ENTEROBACTERIACEAE - 14/02/2017

Articole semnate de acelasi autor in Revista Romana de Boli Infectioase:

EVALUAREA A PATRU TESTE FENOTIPICE PENTRU DETECTAREA CARBAPENEMAZELOR DE TIP OXA-48 LA ENTEROBACTERIACEAE

Romanian Journal of Infectious Diseases | Vol. XIX, No. 4, Year 2016

ISSN 1454-3389 | e-ISSN 2069-6051
ISSN-L 1454-3389
DOI: 10.37897/RJID

National Awards “Science and Research”

NEW! RJID has announced the annually National Award for "Science and Research" for the best scientific articles published throughout the year in the official journal.

ICMJE- Recommendations

Read the Recommendations for the Conduct, Reporting, Editing, and Publication of Scholarly work in Medical Journals.

Promoting Global Health

The published medical research literature is a global public good. Medical journal editors have a social responsibility to promote global health by publishing, whenever possible, research that furthers health worldwide.

Daniela Talapan, Olga Mihaela Dorobăț, Coralia Bleotu, Ioana Aldea, Laura Necula, Dragoş Florea, Gabriel Adrian Popescu, Adrian Streinu-Cercel and Alexandru Rafila

REZUMAT

Enterobacteriaceaele producătoare de carbapenemază au potenţialul de a se răspândi rapid in spitale şi în instituţiile de îngrijire a persoanelor cu dizabilităţi, devenind o problemă majoră de sănătate publică şi pentru controlul infecţiilor. PCR rămâne metoda de referinţă pentru identificarea acestor tulpini, dar este încă scumpă. În acest studiu am evaluat performanţa a patru teste fenotipice pentru identificarea Enterobacteriaceaelor producătoare de carbapenemază tip OXA-48 (Oxacilinază-48). Aceste teste au fost: testul Hodge modificat (MHT), un test care conţine discuri cu meropenem singur sau în combinaţie cu diferiţi inhibitori şi temocilina, un test enzimatic şi unul imunocromatografic. Testele s-au efectuat şi interpretat conform ghidurilor sau recomandărilor producătorilor. Cel mai frecvent microorganism inclus în studiu a fost Klebsiella pneumoniae. Cu o singură excepţie (MHT 87,5%), toate testele au avut o specificitate de 100%. Sensibilitatea a fost de 91,25% pentru MHT, 90% pentru combinaţia de discuri, 95% pentru testul enzimatic şi 82,5% pentru testul imunocromatografic.

Cuvinte cheie: Enterobacteriaceae, carbapenemază OXA-48, teste fenotipice

Full text | PDF

EVALUATION OF FOUR PHENOTYPIC TESTS FOR THE DETECTION OF OXA-48-TYPE CARBAPENEMASE IN ENTEROBACTERIACEAE

ISSN 1454-3389 | e-ISSN 2069-6051
ISSN-L 1454-3389
DOI: 10.37897/RJID

National Awards “Science and Research”

NEW! RJID has announced the annually National Award for "Science and Research" for the best scientific articles published throughout the year in the official journal.

ICMJE- Recommendations

Read the Recommendations for the Conduct, Reporting, Editing, and Publication of Scholarly work in Medical Journals.

Promoting Global Health

Daniela Talapan, Olga Mihaela Dorobăț, Coralia Bleotu, Ioana Aldea, Laura Necula, Dragoş Florea, Gabriel Adrian Popescu, Adrian Streinu-Cercel and Alexandru Rafila

ABSTRACT

Carbapenemase-producing Enterobacteriaceae have the potential to rapid dissemination in healthcare settings, becoming a major infection control and public health concern. PCR remains the reference method
for identifying these strains, but is still expensive. We evaluated the performance of four phenotypic tests for identifying OXA-48-producing (Oxacillinase-48) Enterobacteriaceae. These were modified Hodge test
(MHT), a combination disk test with meropenem alone or in combination with various inhibitors and temocillin, an enzymatic test and an immunochromatographic test. The tests were performed and interpreted according to guidelines or manufacturer’s recommendations. The most frequent microorganism included in the study was Klebsiella pneumoniae. All the tests had 100% specificity, except MHT (87,5%). Sensitivity was 91,25% for MHT, 90% for the combination disk test, 95% for the enzymatic test and 82,50% for the immunochromatographic test.

Keywords: Enterobacteriaceae, OXA-48 carbapenemase, phenotypic tests

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Laura Necula

Latest posts by Laura Necula (see all)

Articole semnate de acelasi autor in Revista Romana de Boli Infectioase:

EVALUAREA A PATRU TESTE FENOTIPICE PENTRU DETECTAREA CARBAPENEMAZELOR DE TIP OXA-48 LA ENTEROBACTERIACEAE

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