Indexed in / abstracted by
Stimati cititori, va reamintim ca autorii primi ai articolelor stiintifice pot acumula 80 de credite EMC in urma publicarii. Daca un articol are mai multi autori, cele 80 de credite [...]
Starting with 2016, The Romanian National Society of Infectious Diseases offers Society’s Prize – for the authors who published the best scientific articles [...]
Tema plagiatului este tot mai mult discutata in ultima vreme. Aparitia unor programe performante de cautare si identificare a similitudinilor intre texte [...]
Spontaneous Generation of Infectious Prion Disease in Transgenic Mice
Juan-Maria Torres, Joaquin Castilla, Belen Pintado, Alfonso Gutierrez-Adan, Olivier Andreoletti, Patricia Aguilar-Calvo, Ana-Isabel Arroba, Beatriz Parra-Arrondo, Isidro Ferrer, Jorge Manzanares and Juan-Carlos Espinosa
We generated transgenic mice expressing bovine cellular prion protein (PrPc) with a leucine substitution at codon 113 (113L). This protein is homologous to human protein with mutation 102L, and its genetic link with Gerstmann–Sträussler–Scheinker syndrome has been established. This mutation in bovine PrPc causes a fully penetrant, lethal, spongiform encephalopathy. This genetic disease was transmitted by intracerebral inoculation of brain homogenate from ill mice expressing mutant bovine PrP to mice expressing wild-type bovine PrP, which indicated de novo generation of infectious poisons. Our findings demonstrate that a single amino acid change in the PrP sequence can induce spontaneous generation of an infectious prion disease that differs from all others identified in hosts expressing the same PrPc sequence. These observations support the view that a variety of infectious prion strains might spontaneously emerge in hosts displaying random genetic PrPc mutations.